H2O2 enhances the anticancer activity of TMPyP4 by ROS-mediated mitochondrial dysfunction and DNA damage

Cancer is one of the diseases that threatens human health and a leading cause mortality worldwide. High levels reactive oxygen species (ROS) have been observed in cancer tissues compared with normal vivo, it not yet known how this influences chemotherapeutic drug action. Cationic porphyrin 5,10,15,20-tetra-(N-methyl-4-pyridyl) (TMPyP4) photosensitizer used photodynamic therapy (PDT) telomerase inhibitor treatment telomerase-positive cancer. Here, we investigated anticancer activity TMPyP4 A549 PANC cells cultured H2O2. The results showed to alone, combination H2O2 exhibited sensitization effects on cell viability colony formation inhibition apoptosis cells, but had no effect MIHA cells. Mechanistically, activates high ROS mitochondrial membrane potential resulting intense DNA damage responses. Consequently, combined upregulates expression BAX, cleaved caspase 3, p-JNK downregulates Bcl-2 Taken together, these data suggested enhanced TMPyP4-mediated ROS-dependent related apoptotic protein regulation, revealing tumor microenvironment plays an important role